Chemistry
Using Fluorescence Anisotropy to Measure Protein-Protein Binding Affinities
Quick fact
Fluorescence anisotropy can determine a protein binding affinity (Kd) in solution without needing to immobilize either protein, making it a powerful tool in drug discovery and studying protein interactions in near-native conditions.
Why this is interesting
You know how a spinning figure skater slows down when they extend their arms? What if I told you that the same principle—rotational inertia—can tell scientists exactly how strongly two proteins stick to each other?