Chemistry
Measuring Binding Enthalpies with Isothermal Titration Calorimetry
Quick fact
ITC is the only technique that directly measures the binding enthalpy (ΔH) of a biomolecular interaction without needing to make assumptions or use fluorescent labels. It records the tiny amounts of heat released or absorbed as one molecule is titrated into another.
Why this is interesting
You've probably heard that proteins bind their partners — but did you know we can measure the heat of that embrace? ITC gives us a direct thermodynamic 'fingerprint' of molecular recognition.