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Chemistry

The Effect of Ionic Strength on the Rate of Enzyme-Catalyzed Reactions

Quick fact

Enzymes with highly charged active sites can show a two- to tenfold change in their reaction rate when the salt concentration in the surrounding solution is raised from very low to moderate (e.g., from 1 mM to 100 mM). This happens because dissolved ions shield the electrostatic attractions and repulsions that guide substrate binding.

Why this is interesting

You’ve probably seen in the lab that adding a pinch of salt can speed up an enzyme reaction—or shut it down completely. What’s going on? That’s the hidden power of ionic strength.